Formulation, characterization and evaluation of silver nano particles of grewia asiatica l. for antimicrobial activity

Ravi Shankar Rao, Vijay Kumar R, Shankrayya M, Chaithra R.P, Kishor Rahul R.P, Pradeep U

Green synthesis of nanoparticles is a novel way to synthesis nanoparticles by using biological sources. It is gaining attention due to its cost effective, eco-friendly and largescale production possibilities. In this present study grewia asiatica leaves extract was taken to investigate the potential for synthesizing silver nanoparticle. The silver nanoparticles synthesized were confirmed by their microscopy change of colour to dark brown due to the phenomenon of surface plasmon resonance. The characterization studied was done by UV-Vis spectroscopy, Scanning electron (SEM), Zeta potential studies and Fourier Transmission infrared spectroscopy (FTIR). The UV absorption peak at 448 nm clearly indicates the formation of AgNPs. The SEM characterization techniques confirmed successful formation of spherical AgNPs Particle size and Zeta potential studies validated the size and charge of the nanoparticles in the colloidal system without any aggregation. Moreover, the biological evaluation of AgNPs revealed good bactericidal and antifungal properties against Staphylococcus aureus, Escherichia coli, and A. niger, C. albicans, which are pathogens commonly involved in infectious of various diseases.

Keywords –Green synthesis, silver nanoparticles, grewia asiatica, SEM